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iPSC Derived Cardiomyocytes

Cardiosight®-S | iPSC Derived Cardiomyocytes

Catalog number	Description	Volume	Add to cart
C-001	NEXEL Cardiosight®-S , small Cardiosight®-S, Cryopreserved hiPSC-derived Cardiomyocytes	≥ 2.5 × 10⁶ Viable cells / Vial	Add to cart
C-002	NEXEL Cardiosight®-S, large Cardiosight®-S, Cryopreserved hiPSC-derived Cardiomyocytes	≥ 5 × 10⁶ Viable cells / Vial	Add to cart
CMS-001	*We recommend on long-term culture Cardiosight®-S Media Kit Cardiosight®-S Media (CM-001, 100 ml) Cardiosight®-S Maintenance Supplement (CS-001, 2 ml, 100X) Cardiosight®-S Plating Supplement (CS-010, 0.6ml, 50X)	100 ml	Add to cart
CMS-002	*We recommend on long-term culture Cardiosight®-S Media Kit Cardiosight®-S Media (CM-002, 200 ml) Cardiosight®-S Maintenance Supplement (CS-001, 2 ml X 2, 100X) Cardiosight®-S Plating Supplement (CS-020, 0.9ml, 50X)	200 ml	Add to cart
CMS-001A	*We recommend on electrophysiological experiments Cardiosight®-S Media Kit Cardiosight®-S Advanced Plating Media (CM-010A, 30 ml) Cardiosight®-S Advanced Maintenance Media (CM-001A, 100 ml) Cardiosight® -S Advanced Plating Supplement (CS-010A, 0.6 ml, 50X) Cardiosight®-S Advanced Maintenance Supplement 1 (CS-001A, 1 ml, 100X)	100 ml	Add to cart
CMS-002A	*We recommend on electrophysiological experiments Cardiosight®-S Media Kit Cardiosight®-S Advanced Plating Media (CM-020A, 45 ml) Cardiosight®-S Advanced Maintenance Media (CM-002A, 200 ml) Cardiosight® -S Advanced Plating Supplement (CS-020A, 0.9 ml, 50X) Cardiosight®-S Advanced Maintenance Supplement 1 (CS-002A, 2 ml, 100X)	200ml	Add to cart
C-001	NEXEL Cardiosight®-S, 小型 Cardiosight®-S, Cryopreserved hiPSC-derived Cardiomyocytes	≥ 2.5 × 10⁶ Viable cells / Vial	Add to cart
C-002	NEXEL Cardiosight®-S, 大型 Cardiosight®-S, Cryopreserved hiPSC-derived Cardiomyocytes	≥ 5 × 10⁶ Viable cells / Vial	Add to cart
CMS-001	Cardiosight®-S Media (CM-001, 100 ml) Cardiosight®-S Maintenance Supplement (CS-001, 2 ml) Cardiosight®-S Plating Supplement (CS-010, 0.6ml)	100 ml	Add to cart
CMS-002	Cardiosight®-S Media (CM-002, 200 ml) Cardiosight®-S Maintenance Supplement (CS-001, 2 ml X 2) Cardiosight®-S Plating Supplement (CS-020, 0.9ml)	200 ml	Add to cart
CMS-001A	Cardiosight®-S Advanced Plating Medium (CM-010A, 30 ml) Cardiosight®-S Advanced Maintenance Medium (CM-001A, 100 ml) Cardiosight® -S Advanced Plating Supplement (CS-010A, 0.6 ml) Cardiosight®-S Advanced Maintenance Supplement 1 (CS-001A, 1 ml)	100 ml	Add to cart
CMS-002A	Cardiosight®-S Advanced Plating Medium (CM-020A, 45 ml) Cardiosight®-S Advanced Maintenance Medium (CM-002A, 200 ml) Cardiosight® -S Advanced Plating Supplement (CS-020A, 0.9 ml) Cardiosight®-S Advanced Maintenance Supplement 1 (CS-002A, 2 ml)	200ml	Add to cart

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产品说明 | iPSC Derived Cardiomyocytes

欢迎您了解 iPSC-derived Cardiomyocytes. NEXEL使用最优化独家协议，致力于提供源自人类诱导性多能干细胞（iPS）的优质人类心肌细胞。Cardiosight®-S是纯粹且由电生理学激活的细胞集合，适合进行心肌细胞领域所有种类的试验。本公司的首要任务是支援研究者获得可信赖的产品，并加快各种项目的研究速度。
本公司的 iPSC-derived Cardiomyocytes经过多数平台确认及优化质量管理流程，以确保客户可以测试使用本公司产品的准确环境。NEXEL的综合测试流程保证在Nanion Cardioexcyte96及Axion Maestro MEA完成细胞测试。本公司的防伪证明书（CoA）记载有准确结果，NEXEL的技术支援组也将与贵公司合作的技术支援组也将与贵公司合作，保证可以在贵公司的实验室提供相同经验。

产品数据 | iPSC Derived Cardiomyocytes

形态

100x

DIV 1
DIV 3
DIV 5
DIV 7
DIV 11
DIV 14

Cardiosight®-S快速融化，从2天后起开始自发搏动，时间越长越稳定（DIV7时45~60bpm）。Cardiosight®-S cardiomyocytes形成一个同步单分子层，加强分析的再现性和准确性。上图是溶解后72小时、1周及2周时间点的12孔培养板状态（100倍放大）。依照Cardiosight®-S使用者指南植入Cardiosight®-S后，为了证明形成了准确的层而将培养密度设置为150000cells/㎠。

免疫染色法

100x
DIV 7

MLC2V
MLC2A
Merged
Connexin43
cTnT
Merged

Cardiosight®-S体现显示细胞的适当收缩机能的一般心室心肌细胞指标和结构性特点。在9mm盖玻片的基底胶植入3万个细胞，凝固前培养7天。在RT以各供应商建议的浓度培养基本抗体1小时，实施免疫细胞化学法。

电生理学-膜片钳

Cardiosight®-S记录自主心律的膜片钳测量，证明适合与Nanion Patchliner并用。细胞成功率较高，钠电流（INa）可变性低。通过TTX（A）可以测定钠电流的容量依赖性阻滞和INa特定效果，通过ATX-II（加强剂）及Ranolazine（阻断剂）也可以轻松观察晚钠电流（Late）。最后，变更APD90时，可以感应到因BayK产生的钙离子通道活性和硝苯地平产生的抑制。该结果证明，Cardiosight®-S可以准确体现离子通道，进行药物学应用时可以轻松使用。查看本公司的海报，有关Nanion Patchliner Dynamite⁸的具体内容请参考这里。这里

电生理学-动态钳

Cardiosight®-S在并用Nanion Patchliner和Dynamite⁸ add-on的动态钳实验中也证明有出色的结果。A情况，在生理温度下使用Cardiosight®-S时，可记录成年人心室类似作用的可能性。B和C情况，使用动态钳时，可以评估钙离子通道活性（BayK）和抑制（硝苯地平）的效果。查看本公司的海报，有关Nanion Patchliner的具体内容请参考这里

电生理学 – CardioExcyte96

在Nanion CardioExcyte 96实验Cardiosight®-S时，证明孔间可变性低。阻抗及EFP信号的高再现性可实现化合物测试（硝苯地平及多非利特）。通过10%介质变化处理提高浓度，取得与预想一致的结果，证明了心肌细胞的特性。查看本公司的海报，有关Nanion CardioExcyte 96的具体内容请参考这里